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Artificial Breeding Techniques In Sheep
Paragon Veterinary Group set up its advanced sheep breeding services in 2000, and is based at Townhead Farm, Newbiggin, Penrith. There are currently a range of techniques being used to facilitate genetic improvement and maximise quality and therefore profitability in the British sheep flock.
Semen Freezing - this is where semen from a ram can be collected via an artificial vagina and frozen in liquid nitrogen (-196oC). The semen can then be kept for up to 10000 years! Well beyond the ram, and the owner’s life-span. Alternatively it can be exported, which is much easier, safer and lower risk of disease transmission than transporting the ram.
Artificial Insemination in sheep involves the collection of semen from a tup and its
subsequent transfer to the reproductive tract of the ewe by artificial means.
There are two main methods: 1. Cervical Insemination -
this is
similar to the method commonly used in cows. Unlike cows the cervix is rarely
penetrated in sheep due to its narrow, tortuous nature. The only way this can
give adequate results with frozen semen is when the ewes are inseminated to a
natural oestrus. This renders it impractical in most management situations. 2. Laparoscopic Insemination
-
This offers
good results with both fresh and
frozen semen. It involves keyhole surgery under sedation and a local
anaesthetic.
To allow
easier management AI in sheep is generally ‘fixed time’. The ewes are
synchronised and the inseminator arrives at a prearranged time to AI the whole
flock. This means that timing is crucial, especially when using frozen semen,
which has a relatively short life span.
Synchronisation generally involves a progesterone-impregnated sponges. To improve the results a
drug called PMSG (a gonadotropin) is also used at varying quantities depending
on breed and season.
The
synchronisation itself represents a major benefit to the farmer allowing much
easier management of feeding ewes
prior to lambing and lambing itself. The subsequent lambs are also easier to
manage.
Other
methods of synchronisation involve melatonin implants (melatonin is a hormone
which controls season in sheep) or teasers (vasectomised males).
Teaser Rams have been vasectomised i.e. a section of their vas deferens (tube carrying semen from testicle to urethra) is removed and the ends tied off. This means that the tup still produces testosterone, has the urge to mount and emits pheromones but ceases to produce fertile semen. Introducing teaser tups in the transitional period of the ewe’s natural season will have the effect of encouraging the ewes to cycle earlier than usual. Teasers should have excellent libido and be a compatible
breed for the time of year their use is intended. They should be carrying plenty
of condition before they are introduced (CS 3.5) and should have a full MOT
(feet, worms, insecticide, multivits.) in plenty of time prior to introduction.
Use
of Teasers in an Ewes should be weaned and separated completely from sight
and smell of a tup for 4-6 weeks
prior to the introduction of teasers in order for this ‘ram effect’ to work.
The teasers are then introduced, ideally at a ratio of 1:25 ewes and remain with them for 2-3 weeks. The sponges are then inserted and the teasers removed.
When the sponges are removed the teasers are re-introduced with raddle on. This
enables the AI operator to assess how well the ewes have responded and also to
avoid using expensive frozen semen in unmarked ewes. Extremely high conception
rates can be achieved if only marked ewes are inseminated.
Use of Teasers in Natural Breeding
Ewes are
weaned and kept separate from any males for 4-6
weeks. The teasers are then introduced for 2 weeks only. The teasers are then replaced with entire rams at a
ratio of 1:25 ewes. 40-60% of ewes
will have a fertile oestrus about 18 days from the introduction of teasers. The
rams can be left in however the preferred method is to leave them in a week with
one colour raddle on, take them out for a week, then reintroduce them with a
different colour raddle on. In commercial ewes one can leave the ram in for a
second period of 2 weeks and then remove him completely. One would then cull any
barreners at scanning time. With pedigree sheep one would probably just leave
the tup in on the first or second occasion depending whether a break in lambing
was required. MOET- this
involves super-ovulating an individual ewe (the DONOR), inseminating her, and
collecting the resulting embryos. The embryos are then transferred into
RECIPIENT or surrogate ewes. The procedure can be carried out several times in
one season, and can also be successful out of season due to the effect of the
drugs used. The resulting embryos can be frozen for transfer at a later date or
transferred ‘fresh’. MOET
can be used to make rapid genetic gain by increasing selection intensity on the
ewe side of the equation. Frozen semen can also be used for the insemination.
Using frozen semen with MOET leads to a greater lamb crop per dose of frozen
semen used. MOET
can also be used as a treatment for infertility. The super-ovulatory drugs, and
the stage at which the embryos are taken means that several forms of infertility
can be avoided. MOET
especially comes into its own when aged ewes which may have a damaged mammary
gland, or who are unable to carry or rear lambs for a variety of other reasons,
can be taken out of production and given a ‘rest’. The embryos she produces
can be transferred into younger, fitter, healthier ewes giving the lambs a much
better chance of survival. Success
of MOET in sheep can be variable just as it can in other species. Factors such
as breed, season, age, condition and certain genetic differences all combine to
produce variability in results. Different ewes respond differently and the same
ewe flushed several times can respond differently. Embryo Freezing - embryos
can be frozen in liquid nitrogen, in a similar manner to semen. This facilitates
transfer of genetic material both within and outwith the UK. This allows access
to markets otherwise difficult to reach for British pedigree sheep farmers. Ovum Pick-Up (OPU) and In-Vitro Fertilisation (IVF) -
this has
been utilised in other species including humans and cattle to great success. It
avoids major surgery and allows a much greater potential harvest of
eggs/embryos.
In sheep the
eggs are harvested from the ovary using a laparoscope in a live animal or taken
from the ovaries recovered from a cadaver. The eggs are matured and fertilised
in a lab and the subsequent embryos transferred to recipient ewes as described
previously. Unlike conventional embryo recovery, eggs can potentially be
collected on a weekly basis and no super-ovulation is required.
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